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Precision Denaturation| Odorless Reduction| Superior Resolution| Western Blot Optimized

What Is LDS Sample Buffer (4X)?

Atheris Bio™ LDS Sample Buffer (4X) is a chemically defined, high-performance buffer designed for denaturation, reduction, and loading of protein samples in SDS-PAGE and Western blot workflows.

Optimized for controlled denaturation at 65°C, this formulation enables efficient protein unfolding with minimal aggregation, improving band clarity and reproducibility—especially for sensitive or complex protein samples.

Its balanced Tris buffering system (pH 6.8 ± 0.05) combined with a low-odor reducing environment and stabilizing additives ensures reliable and consistent sample preparation across experiments.

Quick Product Facts

Key Features

• Optimized Denaturation at 65°C
  Supports uniform protein unfolding with reduced aggregation vs 95°C protocols
• Low-Odor Reducing System (TCEP-Based)
  Stable, user-friendly alternative to traditional reducing agents
• Enhanced Protein Stability
  Helps maintain solubility during denaturation—ideal for sensitive proteins
• Improved Loading Performance (Ficoll + Glycerol)
  Ensures consistent sample density and sharp lane resolution
• Visual Tracking System (Coomassie-Based)
  Enables clear monitoring of sample loading and electrophoresis progress
• Chemically Defined & Reproducible
  Designed for consistent results across experiments and users

Typical Applications

• SDS-PAGE sample preparation
• Western blot analysis
• Protein denaturation workflows
• Cell lysate preparation
• Membrane and cytosolic protein analysis
• Aggregation-prone or sensitive protein samples

Compatibility

✔ Bis-Tris gels (optimized)
✔ Tris-glycine SDS-PAGE gels
✔ Precast gradient gels (TGX systems)

✖ Not suitable for native PAGE

Atheris vs Basic LDS Sample Buffer

Storage & Handling

• Store at 2–8°C
• Mix gently before use
• Avoid contamination
• Use only if solution is clear

Quality Control

Each batch undergoes strict QC to ensure:

• pH verification (6.8 ± 0.05)
• Visual clarity and homogeneity
• Functional electrophoresis performance
• Formulation consistency

Frequently Asked Questions

Is LDS Sample Buffer compatible with standard SDS-PAGE workflows?

Yes, it integrates seamlessly into conventional SDS-PAGE systems.

What gel types work best?

Optimized for Bis-Tris gels, and compatible with Tris-glycine and precast gradient gels.

Is heating required?

Yes, heating at 65°C is recommended for optimal protein denaturation.

Does it replace traditional sample buffers?

Yes, it is a high-performance, user-friendly alternative.

Is this product for diagnostic use?

No. This product is for Research Use Only (RUO).

Defined Denaturation. Reliable Results.