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Sterile | Low Endotoxin | Indicator-Free | Dilution-Validated PBS Concentrate

What Is PBS 10× Concentrate?

PBS 10× Concentrate is a sterile phosphate-buffered saline concentrate designed for reliable preparation of reproducible 1× PBS solutions used in routine laboratory workflows.

Preparing PBS from concentrated stocks is common across research and manufacturing environments. However, variability in dilution technique, water quality, or preparation practices can introduce differences in pH, ionic strength, and osmolality.

PBS 10× Concentrate is formulated as a controlled dilution standard, supporting consistent preparation of physiologic 1× PBS (pH 7.4) when diluted according to the recommended protocol.

The buffer is indicator-free, chemically defined, and endotoxin-controlled, helping laboratories maintain reliable preparation of PBS across users, sites, and workflows.

Quick Product Facts

Product Overview

PBS is one of the most widely used buffers in cell culture and molecular biology workflows. When laboratories prepare 1× PBS from concentrate, variability can occur due to differences in preparation conditions.

Sources of variability may include:

• inconsistent dilution technique
•  variable water quality
•  incomplete mixing
• cross-site preparation differences
• labeling errors in shared laboratories
• batch-to-batch impurity variation
• lack of endotoxin transparency

PBS 10× Concentrate provides a controlled starting material designed to help laboratories produce consistent 1× PBS solutions across experiments and facilities.

Key Features

Dilution-Validated Performance

Designed to support predictable 10× → 1× dilution performance when prepared as directed.

Consistent Final pH

Produces physiologic pH (7.40 ± 0.05) after proper dilution.

Sterile & Low Endotoxin

Sterile-filtered buffer with controlled endotoxin levels suitable for sensitive cell culture workflows.

Indicator-Free & Phenol Red-Free

Clear buffer designed to avoid optical interference in downstream assays.

Designed for Reproducibility

Supports consistent PBS preparation across laboratories, users, and workflows.

Ready-to-Dilute Concentrate

Provided as a 10× concentrate for convenient preparation of working PBS solutions.

Dilution Protocol (10× → 1×)

To prepare 1× PBS:

1. Mix 1 part PBS 10× Concentrate with 9 parts laboratory-grade water

2. Use DI water, Milli-Q water, or WFI-equivalent water

3. Mix thoroughly until fully homogeneous

Validated 1× PBS Performance

When diluted according to the recommended protocol:

Typical Applications

PBS prepared from PBS 10× Concentrate may be used in workflows including:

• cell culture washing buffer preparation
• flow cytometry washing steps
• molecular biology sample preparation
• reagent dilution and preparation
• routine laboratory buffer preparation
• viral vector production support workflows
• QC and analytical laboratory workflows
• high-throughput screening preparation

Researchers should evaluate suitability for their specific experimental systems.

Standard PBS Preparation vs PBS 10× Concentrate

Quality Control

Each batch is manufactured under controlled conditions and tested to ensure consistent performance.

Quality parameters typically include:

• pH verification
• sterility testing
• endotoxin specification
•  visual inspection for clarity
• formulation verification

Refer to lot-specific Certificates of Analysis (COA) for detailed quality information.

Storage & Handling

Store at 2–25 °C.

Avoid prolonged exposure to CO₂ environments.

Do not autoclave the concentrate.

Always dilute to 1× before use with cells.

Frequently Asked Questions

How do I make 1× PBS from PBS 10× Concentrate?

Mix 1 part 10× concentrate with 9 parts laboratory-grade water.

Can I use PBS 10× directly on cells?

No. The buffer must be diluted to 1× before use with cells.

Is PBS 10× the same as DPBS?

PBS and DPBS are similar, but DPBS formulations may include calcium or magnesium depending on the formulation.

Is the final pH controlled after dilution?

Yes. When diluted as directed, final pH is 7.40 ± 0.05.

Is the buffer sterile?

Yes. The product is 0.22 µm sterile filtered.

What water should I use for dilution?

Use DI, Milli-Q, or equivalent laboratory-grade water.

Can I autoclave the concentrate?

No. Do not autoclave the concentrate

Reproducibility Across Users and Sites