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Glycoprotein Optimized | Anti-Smear Formulation | RUO | Stabilized Extraction System

What is RIPA-Glyco Cell Lysis Buffer (1X)?

Atheris Bio™ RIPA-Glyco Cell Lysis Buffer (1X) is a chemically defined lysis buffer engineered for efficient extraction and stabilization of glycoproteins and smear-prone membrane proteins.

Designed specifically for challenging targets, RIPA-Glyco™ helps improve protein integrity, band clarity, and reproducibility in downstream workflows such as Western blot and SDS-PAGE.

Why This Matters

Glycoprotein workflows often suffer from:

• Smearing in Western blot
• Protein aggregation during lysis
• Poor band resolution
• Inconsistent results

RIPA-Glyco™ is designed to reduce these issues, enabling cleaner and more reliable data.

Key Benefits

Smear Reduction Optimized

Helps minimize aggregation and improves band clarity

Designed for Glycoproteins

Supports extraction of heavily modified and membrane-associated proteins

Balanced Extraction System

Efficient lysis while maintaining protein integrity

Stabilized Protein Environment

Supports protein structure during extraction

Reproducible Performance

Chemically defined formulation reduces variability

Ready-to-Use Format

Eliminates inconsistencies from in-house buffer preparation

Typical Applications

• Glycoprotein extraction
• Membrane protein analysis
• Western blot (WB)
• SDS-PAGE workflows
• Post-translational modification studies

Compatibility

✔ Mammalian cell lysates
✔ Adherent and suspension cultures
✔ Western blot and SDS-PAGE workflows

⚠ Not recommended for:

• Enzyme activity assays
• Live-cell applications

Storage & Handling

• Store at 2–8°C
• Do not freeze
• Mix gently before use
• Use aseptic technique
• Stability- 1 month

Compatibility Table

Best  Assay Practice Recommendations

• Use BCA assay for most accurate results
• Prepare standards in the same buffer matrix
• Dilute samples (≥1:5) to minimize interference
• Always include a buffer blank

Quality Control

Each lot is tested for:

• pH verification
• Visual clarity
• Functional performance
• Batch consistency

Frequently Asked Questions

Is this better than standard RIPA for glycoproteins?

Yes, it is specifically optimized to improve stability and reduce smearing.

Can it improve Western blot results?

Yes, it is designed to enhance band clarity and reproducibility.

Do I need to add inhibitors?

Optional depending on your application.

Is it compatible with SDS-PAGE?

Yes, fully compatible.

Is this product for clinical use?

No. For Research Use Only (RUO).

“Stop the smear. Reveal the signal.”