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Co-IP Optimized | Gentle Lysis | RUO | Interaction-Focused Extraction

What is RIPA-Interact  Cell Lysis Buffer (1X)?

Atheris Bio™ RIPA-Interact  Cell Lysis Buffer (1X) is a chemically defined lysis buffer designed for co-immunoprecipitation (Co-IP) and protein–protein interaction studies, where maintaining native interactions is important.

The formulation uses a milder detergent system to reduce disruption of protein complexes during extraction, supporting recovery of interaction partners for downstream analysis.

Key Benefits

• Designed for protein–protein interaction studies
• Gentle lysis conditions compared to standard RIPA buffers
• Supports recovery of complex-associated proteins
• Compatible with Co-IP and downstream Western blot workflows
• Ready-to-use format for workflow consistency

Typical Applications

• Co-immunoprecipitation (Co-IP)
• Protein–protein interaction studies
• Signaling complex analysis

Compatibility

✔ Mammalian cell lysates
✔ Adherent and suspension cultures
✔ Co-IP workflows
✔ Western blot (downstream analysis)

⚠ Not recommended for:

• Strong denaturing lysis requirements
• Maximum membrane protein solubilization
• Enzyme activity assays

Protein Quantification Compatibility

Note: Reduced ionic detergent improves compatibility compared to standard RIPA, though optimization may still be required.

Stability & Storage

Storage & Handling

• Store at 2–8°C
• Do not freeze
• Mix gently before use
• Use aseptic technique

Quality Control

Each lot is tested for:

• pH verification
• Visual clarity
• Functional performance
• Batch consistency

Frequently Asked Questions

How is RIPA-Interact different from RIPA-IP?

RIPA-Interact™ uses gentler lysis conditions designed to help preserve protein interactions.

Can it improve Co-IP results?

It may improve recovery of interaction partners compared to harsher buffers.

Which protein assay should I use?

BCA assay is recommended for consistent results.

Do I need inhibitors?

Yes, recommended based on experimental needs.

Is this product for clinical use?

No. For Research Use Only (RUO).

“Gentle lysis designed to help preserve protein interactions.”