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IP-Optimized | Balanced Lysis | RUO | Consistent Performance

What is RIPA-IP Cell Lysis Buffer (1X)?

Atheris Bio™ RIPA-IP Cell Lysis Buffer (1X) is a chemically defined lysis buffer developed for routine immunoprecipitation (IP) workflows, where consistent protein recovery and assay compatibility are critical.

The formulation provides a balanced lysis environment, enabling efficient protein extraction while maintaining conditions suitable for antibody-based pull-down assays and downstream analysis.

Key Benefits

• Designed for routine immunoprecipitation workflows
• Provides consistent protein extraction across samples
• Supports reliable downstream Western blot analysis
• Balanced detergent system for controlled lysis conditions
• Ready-to-use format to reduce preparation variability

Typical Applications

• Immunoprecipitation (IP)
• Protein enrichment prior to Western blot
• Routine protein analysis workflows

Compatibility

✔ Mammalian cell lysates
✔ Adherent and suspension cultures
✔ IP workflows
✔ Western blot (downstream analysis)

⚠ Not recommended for:

• Weak or transient interaction studies (Co-IP)
• Enzyme activity assays

Protein Quantification Compatibility

Note: Ionic detergent content may affect dye-binding assays. Use appropriate standards and dilution.

Stability & Storage

Storage & Handling

• Store at 2–8°C
• Do not freeze
• Mix gently before use
• Use aseptic technique

Quality Control

Each lot is tested for:

• pH verification
• Visual clarity
• Functional performance
• Batch consistency

Frequently Asked Questions

Can RIPA-IP™ be used for Co-IP?

It may be suitable for strong interactions; gentler buffers are recommended for sensitive complexes.

Is it compatible with Western blot?

Yes, lysates are fully compatible with SDS-PAGE and Western blot workflows.

Which protein assay should I use?

BCA assay is recommended for reliable quantification.

Do I need to add inhibitors?

Yes, protease/phosphatase inhibitors are recommended depending on your application.

Is this product for clinical use?

No. For Research Use Only (RUO).

“Balanced lysis for consistent immunoprecipitation.”