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Membrane Protein Optimized | Receptor Extraction | RUO | Advanced Multi-Detergent System

What Is RIPA-Membrane  Cell Lysis Buffer (1X) ?

Atheris Bio RIPA-Membrane  Cell Lysis Buffer (1X) is a chemically defined, multi-detergent lysis buffer engineered for efficient extraction of membrane proteins, including receptors, ion channels, and transporters.

Unlike standard RIPA buffers, which often underperform for membrane-associated targets, RIPA-Membrane™ incorporates a hybrid detergent system (non-ionic + ionic + zwitterionic + sterol-based) to enhance lipid bilayer disruption while maintaining protein integrity.

The optimized buffering system (HEPES + NaCl) combined with stabilizing components (glycerol, EDTA) supports high-yield membrane protein recovery with improved reproducibility across workflows.

Why This Matters

Membrane proteins are among the most difficult targets to extract, often resulting in:

• Poor receptor recovery
• Incomplete membrane solubilization
• Protein aggregation or loss
• Inconsistent experimental results

RIPA-Membrane is designed to overcome these limitations, enabling reliable extraction of challenging membrane-associated proteins.

Key Benefits

Enhanced Membrane Protein Recovery

Efficient extraction of receptors, transporters, and lipid-associated proteins

Advanced Multi-Detergent System

NP-40 substitute + CHAPS + Digitonin + deoxycholate + low SDS

Balanced Protein Solubilization

Improved membrane disruption without excessive denaturation

Stabilized Extraction Environment

Glycerol preserves protein structure; EDTA inhibits proteases

Reproducible Performance

Chemically defined formulation minimizes batch variability

Ready-to-Use Format

Eliminates inconsistencies from in-house buffer preparation

Typical Applications

• Membrane protein extraction
• GPCR and receptor analysis
• Ion channel studies
• Transporter protein isolation
• Western blot (WB)
• Immunoprecipitation (IP)
• Cell signaling analysis

Compatibility

✔ Mammalian cell lysates
✔ Adherent and suspension cultures
✔ Western blot and proteomics workflows

⚠ Not recommended for:

• Enzyme activity assays
• Native protein function studies requiring minimal detergents
• Live-cell applications

How It Works

• NP-40 substitute disrupts lipid bilayers
• CHAPS preserves protein structure during solubilization
• Digitonin selectively extracts cholesterol-rich membranes
• Sodium deoxycholate + SDS enhance membrane and nuclear protein solubilization
• HEPES buffer maintains stable pH (7.4)
• NaCl supports ionic balance and protein solubility
• EDTA inhibits metal-dependent proteases
• Glycerol stabilizes extracted proteins

Atheris RIPA-Membrane vs Standard RIPA Buffers

Protein Measurement Compatibility

Storage & Handling

• Store at 2–8°C
• Do not freeze
• Mix gently before use
• Use aseptic technique

Quality Control

Each lot is tested for:

• pH verification
• Visual clarity
• Functional lysis performance
• Batch consistency

Frequently Asked Questions

Is RIPA-Membrane different from standard RIPA?

Yes. It is specifically optimized for membrane protein extraction with an advanced detergent system.

Can it improve receptor detection?

Yes, it is designed to enhance recovery of receptors and membrane-associated proteins.

Do I need to add inhibitors?

Optional—protease/phosphatase inhibitors can be added depending on your workflow.

Is it compatible with Western blot?

Yes, fully compatible.

Is this product for clinical use?

No. For Research Use Only (RUO).

Standard RIPA extracts proteins. RIPA-Membrane  extracts the ones you’re missing.