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Efficient Protein Extraction|Western Blot Ready | RUO | Multi-Detergent System 

What Is RIPA-WB  Cell Lysis Buffer ?

Atheris Bio™ RIPA-WB  Cell Lysis Buffer  is a chemically defined, multi-detergent lysis buffer designed for efficient extraction of total cellular proteins across cytosolic, membrane, and nuclear compartments.

Engineered specifically for Western blot workflows, the formulation combines ionic and non-ionic detergents to ensure robust protein solubilization while maintaining downstream compatibility.

The optimized buffering system (Tris + NaCl) and stabilizing components (EDTA, glycerol) support consistent protein recovery, reduced degradation, and improved reproducibility across experiments.

Why This Matters

Protein extraction is often the most variable step in analysis workflows, leading to:

• Incomplete lysis
• Poor membrane protein recovery
• Batch-to-batch variability

RIPA-WB is designed to standardize protein extraction—delivering consistent lysates for reliable downstream analysis.

Key Benefits

• Broad-Spectrum Protein Extraction
  Efficient recovery of cytosolic, membrane, and nuclear proteins
• Optimized for Western Blot
  Balanced detergent system ensures compatibility with SDS-PAGE and blotting
• Multi-Detergent System
  NP-40 substitute + deoxycholate + SDS for complete lysis
• Stabilized Protein Environment
  EDTA inhibits proteases; glycerol improves protein stability
• Reproducible Performance
  Chemically defined formulation reduces variability
• Ready-to-Use Format
  Eliminates inconsistencies from in-house buffer preparation

Typical Applications

• Whole-cell protein extraction
• Membrane protein solubilization
• Western blot sample preparation
• SDS-PAGE workflows
• Cell lysate preparation (adherent and suspension cells)

Compatibility

✔ Mammalian cell lysates
✔ Adherent and suspension cultures
✔ SDS-PAGE and Western blot workflows

⚠ Not recommended for:

• Enzyme activity assays
• Live-cell applications

How It Works

• NP-40 substitute disrupts lipid bilayers
• Sodium deoxycholate + SDS solubilize membrane and nuclear proteins
• Tris buffer maintains stable pH (7.5)
• NaCl supports protein solubility and ionic balance
• EDTA inhibits metal-dependent proteases
• Glycerol stabilizes extracted proteins

Atheris RIPA-WB vs Standard RIPA Buffers

Storage & Handling

• Store at 2–8°C
• Do not freeze
• Mix gently before use
• Use aseptic technique

Quality Control

Each lot is tested for:

• pH verification
• Visual clarity
• Functional lysis performance
• Batch consistency

 Frequently Asked Questions

Is RIPA-WB equivalent to standard RIPA buffer?

Yes, but optimized for reproducibility and Western blot workflows.

Can it extract membrane proteins?

Yes, the multi-detergent system supports efficient membrane protein solubilization.

Do I need to add inhibitors?

Optional—protease/phosphatase inhibitors can be added depending on application.

Is it compatible with SDS-PAGE?

Yes, fully compatible.

Is this product for clinical use?

No. For Research Use Only (RUO).

"Reliable Lysis. Reproducible Western Blots"