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Universal Protein Extraction | Multi-Detergent System | RUO | Stabilized Lysis

What Is Super-RIPA Universal?

Super-RIPA Universal™ Cell Lysis Buffer (1X) is an advanced lysis system designed for comprehensive protein extraction across cytosolic, membrane, and glycosylated targets—using a single, unified buffer.

Engineered with a multi-mechanism solubilization system and protein-stabilizing chemistry, it enables consistent extraction performance without the need for multiple buffer systems.

Why This Matters

Standard workflows often require multiple lysis buffers to cover different protein classes, leading to:

• Workflow complexity
• Inconsistent extraction conditions
• Loss of difficult targets (membrane/glycoproteins)
• Poor reproducibility across experiments

This buffer is designed to simplify workflows while improving extraction coverage and consistency.

Key Benefits

Universal Protein Coverage
Extracts whole-cell, membrane, and modified proteins in one system

Multi-Mechanism Solubilization
Combines multiple extraction modes for difficult targets

Reduced Workflow Complexity
Eliminates the need for buffer switching

Stabilized Protein Environment
Supports solubility and reduces aggregation

Consistent Western Blot Output
Improves band clarity across protein classes

Ready-to-Use Format
Removes variability from in-house preparation

Typical Applications

• Western blot (WB)
• Whole-cell lysate preparation
• Membrane protein extraction
• Glycoprotein workflows
• Comparative proteomics

Compatibility

✔ Mammalian cell lysates
✔ Adherent and suspension cultures
✔ Western blot and SDS-PAGE workflows

⚠ Not recommended for:

• Enzyme activity assays
• Native protein interaction studies (denaturing conditions)

Storage & Handling

• Store at 2–8°C
• Do not freeze
• Mix gently before use
• Use aseptic technique
• Stability: 6–12 months (RUO)

Assay Compatibility

Best Assay Practices

• Use BCA assay for accurate quantification
• Prepare standards in the same buffer matrix
• Dilute samples (≥1:5) to reduce interference
• Always include a buffer blank

Quality Control

Each lot is tested for:

• pH verification
• Visual clarity
• Functional performance
• Batch consistency

Frequently Asked Questions

Do I still need multiple lysis buffers?

No—this buffer is designed to replace multiple systems with a single solution.

Does it work for membrane proteins?

Yes—optimized for difficult and low-solubility targets.

Will it improve Western blot results?

Yes—designed for consistent extraction and improved band clarity.

Do I need inhibitors?

Recommended to add fresh depending on application.

Is this for clinical use?

No. For Research Use Only (RUO).

“One Buffer. Every Target.”